GrippeAllemagneV3, Main, Exploration, bibRecord, 000531

Detection of influenza virus from throat and pharyngeal swabs with a nested duplex light cycler RT-PCR.

Identifieur interne : 000531 ( Main/Exploration ); précédent : 000530; suivant : 000532

Detection of influenza virus from throat and pharyngeal swabs with a nested duplex light cycler RT-PCR.

Auteurs : Michael Koenig [Allemagne] ; Sandra Kosha ; Mark Hickman ; David Heath ; Scott Riddell ; Wade Aldous

Source :

Diagnostic microbiology and infectious disease [ 0732-8893 ] ; 2003.

RBID : pubmed:12742317

Descripteurs français

KwdFr :
- Allemagne, Femelle, Grippe humaine (diagnostic), Grippe humaine (virologie), Humains, Mâle, Orthomyxoviridae (isolement et purification), Pharynx (virologie), RT-PCR (), Sensibilité et spécificité, Virus de la grippe A (isolement et purification), Virus influenza B (isolement et purification), Études de cohortes.
MESH :
- diagnostic : Grippe humaine.
- isolement et purification : Orthomyxoviridae, Virus de la grippe A, Virus influenza B.
- virologie : Grippe humaine, Pharynx.
- Allemagne, Femelle, Humains, Mâle, RT-PCR, Sensibilité et spécificité, Études de cohortes.
Wicri :
- geographic : Allemagne.

English descriptors

KwdEn :
- Cohort Studies, Female, Germany, Humans, Influenza A virus (isolation & purification), Influenza B virus (isolation & purification), Influenza, Human (diagnosis), Influenza, Human (virology), Male, Orthomyxoviridae (isolation & purification), Pharynx (virology), Reverse Transcriptase Polymerase Chain Reaction (methods), Sensitivity and Specificity.
MESH :
- geographic : Germany.
- diagnosis : Influenza, Human.
- isolation & purification : Influenza A virus, Influenza B virus, Orthomyxoviridae.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- virology : Influenza, Human, Pharynx.
- Cohort Studies, Female, Humans, Male, Sensitivity and Specificity.

Abstract

A rapid duplex RT-PCR method was developed using the Roche LightCycler technology for detection of influenza type A and influenza type B viruses. Ninety-seven clinical specimens were analyzed using the Lightcycler method compared with conventional viral culture. Thirty-seven specimens (38.1%) were positive by RT-PCR using matrix protein (MP) primers for influenza A or B virus, compared to thirteen culture positive specimens (13.4%). All culture positive specimens were also positive by RT-PCR. A nested PCR reaction using hemagglutination (HA) gene primers confirmed all of the earlier positive PCR reactions. This LightCycler PCR technique was found to be more sensitive than viral culture for influenza virus detection.

DOI: 10.1016/s0732-8893(02)00552-7
PubMed: 12742317

Affiliations:

Allemagne

Links toward previous steps (curation, corpus...)

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Le document en format XML

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<author><name sortKey="Koenig, Michael" sort="Koenig, Michael" uniqKey="Koenig M" first="Michael" last="Koenig">Michael Koenig</name>
<affiliation wicri:level="1"><nlm:affiliation>Landstuhl Regional Medical Center, Dept. of Pathology, Microbiology Division, Bldg. 3738, Room 204, Auf dem Kirchberg, Landstuhl 66849, Germany. michael.koenig@lnd.amedd.army.mil</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Landstuhl Regional Medical Center, Dept. of Pathology, Microbiology Division, Bldg. 3738, Room 204, Auf dem Kirchberg, Landstuhl 66849</wicri:regionArea>
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<wicri:noRegion>Landstuhl 66849</wicri:noRegion>
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<author><name sortKey="Kosha, Sandra" sort="Kosha, Sandra" uniqKey="Kosha S" first="Sandra" last="Kosha">Sandra Kosha</name>
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<author><name sortKey="Hickman, Mark" sort="Hickman, Mark" uniqKey="Hickman M" first="Mark" last="Hickman">Mark Hickman</name>
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<author><name sortKey="Heath, David" sort="Heath, David" uniqKey="Heath D" first="David" last="Heath">David Heath</name>
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<author><name sortKey="Riddell, Scott" sort="Riddell, Scott" uniqKey="Riddell S" first="Scott" last="Riddell">Scott Riddell</name>
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<author><name sortKey="Aldous, Wade" sort="Aldous, Wade" uniqKey="Aldous W" first="Wade" last="Aldous">Wade Aldous</name>
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<term>Influenza B virus (isolation & purification)</term>
<term>Influenza, Human (diagnosis)</term>
<term>Influenza, Human (virology)</term>
<term>Male</term>
<term>Orthomyxoviridae (isolation & purification)</term>
<term>Pharynx (virology)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term>
<term>Sensitivity and Specificity</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Allemagne</term>
<term>Femelle</term>
<term>Grippe humaine (diagnostic)</term>
<term>Grippe humaine (virologie)</term>
<term>Humains</term>
<term>Mâle</term>
<term>Orthomyxoviridae (isolement et purification)</term>
<term>Pharynx (virologie)</term>
<term>RT-PCR ()</term>
<term>Sensibilité et spécificité</term>
<term>Virus de la grippe A (isolement et purification)</term>
<term>Virus influenza B (isolement et purification)</term>
<term>Études de cohortes</term>
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<keywords scheme="MESH" type="geographic" xml:lang="en"><term>Germany</term>
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<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Influenza, Human</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Grippe humaine</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A virus</term>
<term>Influenza B virus</term>
<term>Orthomyxoviridae</term>
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<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Orthomyxoviridae</term>
<term>Virus de la grippe A</term>
<term>Virus influenza B</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Grippe humaine</term>
<term>Pharynx</term>
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<term>Female</term>
<term>Humans</term>
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<term>Sensitivity and Specificity</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Allemagne</term>
<term>Femelle</term>
<term>Humains</term>
<term>Mâle</term>
<term>RT-PCR</term>
<term>Sensibilité et spécificité</term>
<term>Études de cohortes</term>
</keywords>
<keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Allemagne</term>
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<front><div type="abstract" xml:lang="en">A rapid duplex RT-PCR method was developed using the Roche LightCycler technology for detection of influenza type A and influenza type B viruses. Ninety-seven clinical specimens were analyzed using the Lightcycler method compared with conventional viral culture. Thirty-seven specimens (38.1%) were positive by RT-PCR using matrix protein (MP) primers for influenza A or B virus, compared to thirteen culture positive specimens (13.4%). All culture positive specimens were also positive by RT-PCR. A nested PCR reaction using hemagglutination (HA) gene primers confirmed all of the earlier positive PCR reactions. This LightCycler PCR technique was found to be more sensitive than viral culture for influenza virus detection.</div>
</front>
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<DateCompleted><Year>2003</Year>
<Month>07</Month>
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<DateRevised><Year>2019</Year>
<Month>09</Month>
<Day>06</Day>
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<Issue>1</Issue>
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<Title>Diagnostic microbiology and infectious disease</Title>
<ISOAbbreviation>Diagn. Microbiol. Infect. Dis.</ISOAbbreviation>
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<Pagination><MedlinePgn>35-7</MedlinePgn>
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<Abstract><AbstractText>A rapid duplex RT-PCR method was developed using the Roche LightCycler technology for detection of influenza type A and influenza type B viruses. Ninety-seven clinical specimens were analyzed using the Lightcycler method compared with conventional viral culture. Thirty-seven specimens (38.1%) were positive by RT-PCR using matrix protein (MP) primers for influenza A or B virus, compared to thirteen culture positive specimens (13.4%). All culture positive specimens were also positive by RT-PCR. A nested PCR reaction using hemagglutination (HA) gene primers confirmed all of the earlier positive PCR reactions. This LightCycler PCR technique was found to be more sensitive than viral culture for influenza virus detection.</AbstractText>
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Detection of influenza virus from throat and pharyngeal swabs with a nested duplex light cycler RT-PCR.

Detection of influenza virus from throat and pharyngeal swabs with a nested duplex light cycler RT-PCR.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki